cd68 clone fa 11 rat monoclonal antibody  (Bio-Rad)

Journal: Cell reports

Article Title: SIRT2 suppresses aging-associated cGAS activation and protects aged mice from severe COVID-19

doi: 10.1016/j.celrep.2025.115562

Figure Lengend Snippet: Young (5 months old) or old (24 months old) mice were inoculated intranasally with control or ORF3a-expressing AAV6. Mice were analyzed 3 weeks later. n =2 independent experiments. (A and B) Quantitative real-time PCR analyses of the mRNA levels of the indicated genes in BALF cells of young and old mice. n = 8. (C) Quantitative real-time PCR analyses of the mRNA levels of IFNβ in the lungs of young mice. n = 6. (D and E) H&E staining (D) and quantification (E) of lung sections of young mice. Scale bar: 100 μm n = 6 mice/group, 10–15 images examined from 3 slides/mouse. (F) Flow cytometry analysis of myeloid cells (Gr1 + CD11b + ) in BALF of young mice. n = 6. (G and H) Immunostaining for Ly6G + cells (G) and quantification (H) of lung sections. Scale bar: 100 μm n = 8 mice/group, 6 images examined from 3 slides/mouse. (I and J) Immunostaining for CD68 + cells (I) and quantification (J) of lung sections. Scale bar: 100 μm n = 8 mice/group, 4 images examined from 3 slides/mouse. (K and L) Sirius red staining (K) and quantification (L) of lung sections. Scale bar: 200 μm n = 6 mice/group, 10 images examined from 3 slides/mouse. (M and N) Immunostaining for phosphorylated STING (M) and quantification (N) of lung sections of young and old mice. Scale bar: 30 μm n = 8 mice/group, 5 images examined from 3 slides/mouse. Data are mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001. See also – .

Article Snippet: CD68 clone FA-11 Rat monoclonal antibody , Bio-Rad , Cat# MCA1957GA; RRID:AB_324217.

Techniques: Control, Expressing, Real-time Polymerase Chain Reaction, Staining, Flow Cytometry, Immunostaining

Journal: STAR Protocols

Article Title: Studying macrophage activation in immune-privileged lens through CSF-1 protein intravitreal injection in mouse model

doi: 10.1016/j.xpro.2021.101060

Figure Lengend Snippet: Subtypes of macrophages in the posterior lens 24 h after CSF-1 intravitreal injection Cryosections of the whole eyeball from dKO and wild type mice were immunostained with antibodies against MΦ (CD68, red), MΦ1 (iNOS, purple), and MΦ2 (Arg-1, green) 24 h after CSF-1 intravitreal injection. OD (OculusDexter) indicates the right eye and OS (OculusSinister) indicates the left eye. Scale bar = 200 μm.

Article Snippet: Rat Anti-Mouse CD68 Monoclonal Antibody | Clone: FA-11 , Bio-Rad Laboratories (Hercules, CA, USA) , MCA1957.

Techniques: Injection

Journal: STAR Protocols

Article Title: Studying macrophage activation in immune-privileged lens through CSF-1 protein intravitreal injection in mouse model

doi: 10.1016/j.xpro.2021.101060

Figure Lengend Snippet: Quantification of macrophages in posterior lens 24 h after CSF-1 intravitreal injection Total numbers (CD68+), MΦ1 (iNOS+), and MΦ2 (Arg-1+) in the posterior regions were quantified 24 h after CSF-1 injection. All the data are presented as mean ± SD. n = 3 per group. ∗∗∗∗, P < 0.0001.

Article Snippet: Rat Anti-Mouse CD68 Monoclonal Antibody | Clone: FA-11 , Bio-Rad Laboratories (Hercules, CA, USA) , MCA1957.

Techniques: Injection

Journal: STAR Protocols

Article Title: Studying macrophage activation in immune-privileged lens through CSF-1 protein intravitreal injection in mouse model

doi: 10.1016/j.xpro.2021.101060

Figure Lengend Snippet:

Article Snippet: Rat Anti-Mouse CD68 Monoclonal Antibody | Clone: FA-11 , Bio-Rad Laboratories (Hercules, CA, USA) , MCA1957.

Techniques: Recombinant, Plasmid Preparation, Double Knockout, Knock-Out, Software, Microscopy